Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509) from QED Bioscience Inc.

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ICC

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.
IHC

Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
IHC

Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot

Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Supplier Page from
QED Bioscience Inc. for
Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509)

QED Bioscience Inc.'s Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509) is a Mouse monoclonal antibody. The Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509) was generated using HCN4 as the antigen. It reacts with Homo Sapiens, Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Amplification, Cell Sorting, Isolation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.

ICC

IHC

IHC

Western Blot

Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509) from QED Bioscience Inc.

QED Bioscience Inc.

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Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509) from QED Bioscience Inc.

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